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Application
Best choice
Alternatives
Germline characterization with no concern for sample crosstalk
i.e., SNPs, indels, crude CNVs
i.e., SNPs, indels, crude CNVs
UDI
SI, CDI, or UDI-UMI
Germline characterization where sample cross talk is important
i.e., translational research, inherited genetics, infectious disease
i.e., translational research, inherited genetics, infectious disease
UDI
UDI-UMI
Low-level mutation detection, down to ~1% frequency
i.e., cancer, mosaicism, other diseases where somatic mutations are important
i.e., cancer, mosaicism, other diseases where somatic mutations are important
UDI-UMI
TruSeq™–Compatible Full-length Adapters
TruSeq™–Compatible Stubby Adapter
TruSeq™– or Nextera™–Compatible Indexing Primers
Delivered as
Duplex
Duplex
Forward and reverse primer mix
Normalized to
15 μM
15 μM
20 μM (10 μM each primer)
Volume delivered
16 reaction
80 μl
80 μl
80 μl
96 reaction
480 μl
480 μl
480 μl
2 nmol
130 μl
130 μl
200 μl
8 nmol
530 μl
530 μl
800 μl
24 nmol
1600 μl
1600 μl
2400 μl
Suggested amount/reaction to use (may vary by application)
75 pmol in 5 μl
75 pmol in 5 μl
50 pmol (per primer) in 5 μl
Introduction
IDT understands that the needs of genomics projects are not one-size-fits-all, and provides custom adapters that you can tailor to your needs. Our scientists have created this Custom Adapter Configurator tool to guide you through selection of several options as part of the adapter design process.
Consecutive, starting with index #1 and ending with index #
Consecutive, starting with index # and ending with index #
NOTE: Index values should be between and
File: ( indexes)
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Source
# Available
Index length
Blocker
Version
View indexes
ILMN
Up to 384
10 bp
Custom
Index upload
Index upload instructions
- When ordering UDI-UMI - Do not include UMI sequences in the upload file. 9bp UMIs are automatically added to your sequence adjacent to the i7.
- Please contact Scientific Application Support if you need to add specific UMI sequences
- If spreadsheet contains more than one worksheet, the worksheet with import data must be named "Sheet1"
- File can contain no more than 2000 indexes
-
Spreadsheet should contain header columns in Row 1 and have the following values in the following spreadsheet cells:
- A1: IndexName
- B1: I5Name (not used)
- C1: I5Barcode (not used)
- B1: I5Name
- C1: I5Barcode
- D1: I7Name
- E1: I7Barcode
The current browser does not support this file upload feature. The following browsers are recommended for this feature:
- IE >= 10
- Firefox >= 3.6
- Chrome >= 13
- Safari >= 6
- Opera >= 11.1
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Upload these indexes?
IndexName
I5Name
I5Barcode
I7Name
I7Barcode
$ 
Add
Summary
Your price for this order: $
Adapter details
What you will receive
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Note: We suggest to avoid using custom adapters (such as those through the Custom Adapter Configurator Tool) and off-the-shelf, stocked adapters together in the same project. Please contact us for further assistance.
Need your adapters sooner? Check out our selection of pre-configured xGen™ UDI-UMI Adapters and xGen™ Stubby Adapter and UDI Primer Pairs in single-use plates.
RUO22-1362_001