IDT will be closed Tuesday, December 24th and Wednesday the 25th for the Christmas holiday and on Tuesday, December 31st through Wednesday, January 1st for the New Year. Normal business hours will resume on Thursday, January 2nd.
5’ Phosphorylation is needed if an oligo is used as a substrate for DNA ligase. 3’ Phosphorylation will inhibit degradation by some 3’-exonucleases and can be used to block extension by DNA polymerases.